Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens. We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone containment Level 3 facility where samples for highly contagious pathogen are received and processed.


All Bacteria Virus Fungi Parasite


Validated
Assay Code See Below
Description Mycobacterium is a genus of Actinobacteria, family Mycobacteriaceae. Over 190 species are recognized in this genus. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans. Bovine tuberculosis is a chronic disease of animals and humans caused by Mycobacterium bovis.

Tuberculosis (TB) is caused by a bacterium called Mycobacterium tuberculosis. The bacteria usually attack the lungs, but TB bacteria can attack any part of the body such as the kidney, spine, and brain. Not everyone infected with TB bacteria becomes sick. As a result, two TB-related conditions exist: latent TB infection (LTBI) and TB disease. If not treated properly, TB disease can be fatal. There are two kinds of tests that are used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests. A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria. It does not tell whether the person has latent TB infection (LTBI) or has progressed to TB disease. Other tests, such as a chest x-ray and a sample of sputum, are needed to see whether the person has TB disease.

Pathogens Tested
  • HPM-038 : Mycobacterium species (This assay includes the detection of Mycobacterium species. This assay can detect M. austroafricanum, M.avium subsp. avium,M.bovis BCG, M.chelonae, M.gordonae, M.fortuitum subsp.fortuitum, M.insubricum, M.intracellulare, M.kansasii, M.marium, M.mucogenicum, M.peregrinum, M.porcinum, M.scrofulaceum, M.setense, M.simiae, M.smegmatis, M.terrae, M.tuberculosis, M.ulcerans, M.xenopi)
  • HPM-100 : Mycobacterium tuberculosis (This assay includes the detection of Mycobacterium species and Mycobacterium tuberculosis.)

Method Real-Time PCR.
Sample Type
For Mycobacterium species: EDTA blood, Stool, Swab / Secretion (Respiratory), Tissue(Biopsy), Culture, CSF, Urine (for urinary TB).
For Mycobacterium tuberculosis: Swab / Secretion (Respiratory), Tissue(Biopsy), CSF, Urine (for urinary TB)
Transport Condition Samples should be transported at 4°C. Stool should be transported to MBG Lab within 24h of collection.
Turn Around Time (TAT) TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.

Links

Validated
Assay Code HPM-041
Description Mycoplasma refers to a genus of bacteria that are the smallest living cells known. They can be parasitic or saprotrophic.

Mycoplasma pneumoniae causes the disease mycoplasma pneumonia, a form of atypical bacterial pneumonia, and is related to cold agglutinin disease. It is also referred to as walking pneumonia. Symptoms include cough that may come in violent spasms but produce very little mucus, mild flu-like symptoms such as fever and chills, sore throat, headache, tiredness malaise and community-acquired pneumonia (CAP). It is spread by contact with droplets from nose and throat of an infected person.

Mycoplasma genitalium is a small parasitic bacterium that lives on cilated epithelial cells of the genital tract and is sexually transmitted. In women symptoms such as vaginal itching, burning while urinating, discharge, pain during intercourse may appear. In the long term, this infection is suspected to cause pelvic inflammatory disease and cervicitis. In men, it is a major agent in urogenital tract disease.

Pathogens TestedThis assay can detect many Mycoplasma species including Mycoplasma agassizii,Mycoplasma anatis, Mycoplasma anseris, Mycoplasma arginini,Mycoplasma arthritidis,Mycoplasma auris, Mycoplasma buccale, Mycoplasma canadense,Mycoplasma cloacale,Mycoplasma collis, Mycoplasma columborale, Mycoplasma cricetuli, Mycoplasma cynos,Mycoplasma falconis,Mycoplasma faucium, Mycoplasma felis, Mycoplasma gateae,Mycoplasma gypis, Mycoplasma hominis, Mycoplasma hyopharyngis, Mycoplasma hyorhinis,Mycoplasma hyosynoviae, Mycoplasma iguana, Mycoplasma lagogenitalium, Mycoplasma leonicaptivi, Mycoplasma molare, Mycoplasma mustelae, Mycoplasma neophronis,Mycoplasma neurolyticum, Mycoplasma orale, Mycoplasma phocicerebrale, Mycoplasma phocidae, Mycoplasma salivarium, Mycoplasma spumans, Mycoplasma timone, and Mycoplasma zalophi .

The assay cannot detect Mycoplasma pneumoniae, Mycoplasma gallisepticum and Mycoplasma pulmonis.

Method Real-Time PCR
Sample Type
Tissue, EDTA blood, Swab/ Secretion (Respiratory), Swab / Secretion (Conjunctival), Swab/ Secretion (Genital), CSF, Culture.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.

Links




Molecular Biology & Genomics Centre

Pathogen Identification


Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens. We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone containment Level 3 facility where samples for highly contagious pathogen are received and processed.


All Bacteria Virus Fungi Parasite


Validated
Assay Code See Below
Description Mycobacterium is a genus of Actinobacteria, family Mycobacteriaceae. Over 190 species are recognized in this genus. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis (Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae) in humans. Bovine tuberculosis is a chronic disease of animals and humans caused by Mycobacterium bovis.

Tuberculosis (TB) is caused by a bacterium called Mycobacterium tuberculosis. The bacteria usually attack the lungs, but TB bacteria can attack any part of the body such as the kidney, spine, and brain. Not everyone infected with TB bacteria becomes sick. As a result, two TB-related conditions exist: latent TB infection (LTBI) and TB disease. If not treated properly, TB disease can be fatal. There are two kinds of tests that are used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests. A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria. It does not tell whether the person has latent TB infection (LTBI) or has progressed to TB disease. Other tests, such as a chest x-ray and a sample of sputum, are needed to see whether the person has TB disease.

Pathogens Tested
  • HPM-038 : Mycobacterium species (This assay includes the detection of Mycobacterium species. This assay can detect M. austroafricanum, M.avium subsp. avium,M.bovis BCG, M.chelonae, M.gordonae, M.fortuitum subsp.fortuitum, M.insubricum, M.intracellulare, M.kansasii, M.marium, M.mucogenicum, M.peregrinum, M.porcinum, M.scrofulaceum, M.setense, M.simiae, M.smegmatis, M.terrae, M.tuberculosis, M.ulcerans, M.xenopi)
  • HPM-100 : Mycobacterium tuberculosis (This assay includes the detection of Mycobacterium species and Mycobacterium tuberculosis.)

Method Real-Time PCR.
Sample Type
For Mycobacterium species: EDTA blood, Stool, Swab / Secretion (Respiratory), Tissue(Biopsy), Culture, CSF, Urine (for urinary TB).
For Mycobacterium tuberculosis: Swab / Secretion (Respiratory), Tissue(Biopsy), CSF, Urine (for urinary TB)
Transport Condition Samples should be transported at 4°C. Stool should be transported to MBG Lab within 24h of collection.
Turn Around Time (TAT) TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.

Links

Validated
Assay Code HPM-041
Description Mycoplasma refers to a genus of bacteria that are the smallest living cells known. They can be parasitic or saprotrophic.

Mycoplasma pneumoniae causes the disease mycoplasma pneumonia, a form of atypical bacterial pneumonia, and is related to cold agglutinin disease. It is also referred to as walking pneumonia. Symptoms include cough that may come in violent spasms but produce very little mucus, mild flu-like symptoms such as fever and chills, sore throat, headache, tiredness malaise and community-acquired pneumonia (CAP). It is spread by contact with droplets from nose and throat of an infected person.

Mycoplasma genitalium is a small parasitic bacterium that lives on cilated epithelial cells of the genital tract and is sexually transmitted. In women symptoms such as vaginal itching, burning while urinating, discharge, pain during intercourse may appear. In the long term, this infection is suspected to cause pelvic inflammatory disease and cervicitis. In men, it is a major agent in urogenital tract disease.

Pathogens TestedThis assay can detect many Mycoplasma species including Mycoplasma agassizii,Mycoplasma anatis, Mycoplasma anseris, Mycoplasma arginini,Mycoplasma arthritidis,Mycoplasma auris, Mycoplasma buccale, Mycoplasma canadense,Mycoplasma cloacale,Mycoplasma collis, Mycoplasma columborale, Mycoplasma cricetuli, Mycoplasma cynos,Mycoplasma falconis,Mycoplasma faucium, Mycoplasma felis, Mycoplasma gateae,Mycoplasma gypis, Mycoplasma hominis, Mycoplasma hyopharyngis, Mycoplasma hyorhinis,Mycoplasma hyosynoviae, Mycoplasma iguana, Mycoplasma lagogenitalium, Mycoplasma leonicaptivi, Mycoplasma molare, Mycoplasma mustelae, Mycoplasma neophronis,Mycoplasma neurolyticum, Mycoplasma orale, Mycoplasma phocicerebrale, Mycoplasma phocidae, Mycoplasma salivarium, Mycoplasma spumans, Mycoplasma timone, and Mycoplasma zalophi .

The assay cannot detect Mycoplasma pneumoniae, Mycoplasma gallisepticum and Mycoplasma pulmonis.

Method Real-Time PCR
Sample Type
Tissue, EDTA blood, Swab/ Secretion (Respiratory), Swab / Secretion (Conjunctival), Swab/ Secretion (Genital), CSF, Culture.
Transport Condition Samples should be transported at 4°C.
Turn Around Time (TAT) TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.

Links